LIFA熒光壽命系統(tǒng)附件
簡 述：
LIFA是適用于熒光壽命顯微成像（FLIM）。該設(shè)備可以利用頻域技術(shù)在任何寬場顯微熒光成像系統(tǒng)獲得壽命變化相關(guān)圖像。系統(tǒng)包含TRiCAM?M可調(diào)制型像增強CCD相機，可調(diào)制光源，調(diào)制控制器，定制電腦以及LI-FLIM軟件。像增強相機包含像增強器，CCD相機，可以獲得*高增益和采集速率。
品 牌：
荷蘭 Lambert Instruments
產(chǎn)品型號：
- LIFA
LIFA熒光壽命系統(tǒng)附件
LIFA是適用于熒光壽命顯微成像（FLIM）。該設(shè)備可以利用頻域技術(shù)在任何寬場顯微熒光成像系統(tǒng)獲得壽命變化相關(guān)圖像。系統(tǒng)包含TRiCAM M可調(diào)制型像增強CCD相機，可調(diào)制光源，調(diào)制控制器，定制電腦以及LI-FLIM軟件。像增強相機包含像增強器，CCD相機，可以獲得*高增益和采集速率。
LIFA熒光壽命系統(tǒng)附件關(guān)鍵特點：
精準的壽命成分：0-300ns的熒光壽命，精度可達30ps RMS
系統(tǒng)技術(shù)參數(shù)：
控制器參數(shù)：控制器可以經(jīng)USB線LI-FLIM軟件控制
多LED光源
多激光光源
TRICAM M相機像增強器指標
CCD相機指標
LIFA是適用于熒光壽命顯微成像（FLIM）。該設(shè)備可以利用頻域技術(shù)在任何寬場顯微熒光成像系統(tǒng)獲得壽命變化相關(guān)圖像。系統(tǒng)包含TRiCAM M可調(diào)制型像增強CCD相機，可調(diào)制光源，調(diào)制控制器，定制電腦以及LI-FLIM軟件。像增強相機包含像增強器，CCD相機，可以獲得*高增益和采集速率。
LIFA熒光壽命系統(tǒng)附件關(guān)鍵特點：
精準的壽命成分：0-300ns的熒光壽命，精度可達30ps RMS
系統(tǒng)技術(shù)參數(shù)：
| Lifetime range | 0 - 300 ns |
| Lifetime resolution | less than 100 ps (single pixel) |
| Speed | 2 lifetime images per second (full frame) |
| Spatial resolution | 26 lp/mm (Gen II); 19 lp/mm (Gen III) |
| Sensitivity | single photon |
| Intrascene dynamic range | 12 bit |
| Camera input format | 1 inch |
| Effective pixel size | 10.3 μm square (12,9 for lens coupled CCD) |
| Signal shape | Sinusoidal |
| Frequency range | 1 MHz – 120 MHz |
| Frequency adjustment resolution | 1 kHz |
| Frequency deviation | 100 ppm max. |
| Harmonics suppression | Better than 25 dB |
| Output 1 Type a: MultiLED AC signal level DC current adjust |
Capable of driving lightsource type a, optionally b and c Adjustable in 250 steps 0 – 1000 mA, 12 bit resolution |
| Optional lightsource outputs: Type b: Modulated laser diode AC signal level DC level adjust Type c: AOM for CW laser AC signal level Frequency Output 2 AC signal level AC signal after amplification |
0.00 – 1.00 V peak-peak 0.00 – 1.00 V 0.00 – 1.00 V peak-peak Half the frequency of output 2 To image intensifier Adjustable from –50 to 0 dBm 15 Vpp nom. |
| External trigger input | LVTTL (phase shift on falling edge or rising edge) |
| Impedance | 50 Ω |
| Phase adjustment range | 0 - 360° in 1° steps |
| Phase deviation | < 0.01° |
| Cathode voltage DC | Adjustable from –150 V to +15 V |
| MCP voltage (gain) DC | Adjustable from 450 – 1000 V max. |
| Anode voltage DC | Fixed 6 kV |
| Peak wavelengths (nm), approximately | 405 (CFP), 445 (cerulean, CFP), 470 (GFP), 535 (TRITC), 630 (CY5), others on request |
| Number of mounted LEDs | 3 (standard), or 4 (maximum) |
| Modulation frequency | up to at least 80 MHz |
| LEDs switching time | 200ms |
| Coupling | Compatible with existing Hg and Xe microscope adapters |
| Lifetime drift | less than 30 ps* |
| Optional Temperature stabilization for long-term (hours) lifetime stability |
Active thermoelectric water cooling/heating system |
| Control temperature range | 5 – 45º C |
| Temperature stability (constant load, constant ambient temp) |
± 0.1º C |
| Dimensions (l x w x h) | 346 x 109 x 183 mm |
| * Measured using stable fluorescent material with single lifetime component of 3.2 ns | |
| Wavelengths (nm) | 375 (BFP), 405 (CFP), 445 (cerulean, CFP), 488 (GFP), 515 (YFP, Venus), 647 (CY5), others on request |
| Number of laser lines | Standard: up to 3 or up to 6; Extreme: up to 4. All combined into one optical fiber output |
| Output power (mW) | 20 mW-300 mW, depending on the wavelength |
| Modulation frequency | Up to 120 MHz (standard) |
| Input signal | Digital: 0...1 V / 50 Ω |
| Control interface | RS232 |
| Coupling | KineFLEX system, with a single-mode polarization maintaining fiber and either FC-APC, FC-PC or FC-P8 output connector |
| Power stability | Standard: 2%*, Extreme: 0.5%* |
| Lifetime drift | Standard: less than 50 ps*, Extreme: less than 20-30 ps* |
| Dimensions (l x w x h) | 19" rack housing. MultiLASER-3: 480 x 484 x 88 mm; MultiLASER-4 and 6: 610 x 484 x 132 mm |
| Options | • Multi-channel excitation • Two single-mode optical fiber outputs • Custom fiber outputs on special request |
| * Measured using stable fluorescent material with single lifetime component of 3.2 ns | |
CCD相機指標
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